Bio-One Of Orange decontamination and biohazard cleaning services

Learning about the 4 Biosafety Levels + How Bio-One of Orange specialists can help you!

Cleaning and decontaminating biohazardous waste is a task that must be done solely by professionals. Work environments involving biological waste, such as laboratories, medical facilities, and chemical labs follow strict guidelines in terms of adequate PPE, and practices to ensure that every material that is handled inside these areas doesn’t spread outside the parameters.


What is Biosafety? 


Every safety precaution that is applied in work environments to reduce employees ’ exposure to potentially infectious microorganisms and bacteria is considered biosafety. Bacteria and airborne pathogens can easily result in disease outbreaks if agencies, facilities, and employers fail to follow these regulations.


Bio-One of Orange works with law enforcement and local authorities to effectively respond in situations where biohazardous waste needs to be contained. Bio-One’s ultimate goal is to protect the community from these dangerous pathogens. 

Bio-One certified technicians follow the must strict and rigoruous procedures to keep you safe!

4-Level Biosafety Classification - How does it work?

There are four levels of biosafety practices. Each level builds upon the other, starting from Biosafety Level 1 (BSL-1)  which represents the “lowest” risk of disease, infection, and potential biohazard, whereas Biosafety Level 4 entails the most rigorous practices that must be followed to ensure biological safety. Laboratories and work environments classified in a Level 4 (BSL-4) are those that handle the most dangerous and exotic bacteria/pathogens, the outbreak of these materials can be fatal, and mostly impossible to treat with medication or vaccination.


Biosafety Level 1(BSL-1) 


Microbes with the lowest or unknown risk of consistently causing diseases in healthy adults. Workplaces designated with BLS-1 classification usually present minimal risk to lab facilities and environments.


Even so, workers must adhere to the following regulations and safety practices:


-Adequate Personal Protective Equipment (PPE) - lab coats, gloves, mask, eye protection. 

-A sink must be available for hand washing/disinfection.

-The lab area should have doors that separate this workspace from the rest of the facility.


Facilities designated with BSL-1 classification include: Research labs with the use of benches. These environments are not isolated from the rest of the facility.

Bio-One specialists work to clean and sanitize commercial properties from biohazardous material.

Biosafety Level 2 (BSL-2)


Built upon BSL-1, environments designated with a BLS-2 classification work with microbes that pose a moderate risk of exposure. Microbes are typically indigenous (microorganisms that have been adapting and surviving within the native soil environments throughout the years) and the intensity of the associated diseases may variate.


Regulations and safety practices include:


-Adequate Personal Protective Equipment (PPE) - lab coats, gloves, mask, eye protection. Emphasis on eye protection and face shields, if necessary.

-Use of Biological Safety Cabinet (BSC) for procedures that may cause splash or airborne transmission.

-A sink and eyewash must be available for disinfection.

-Use of autoclave or specific alternative methods to dispose of biological waste.


Facilities designated with BSL-2 classification include: Laboratories with self-closing doors. While they’re not isolated from the rest of the facility, entrance is restricted when procedures are being conducted.

Biosafety Level 3 (BSL-3) 


Built upon BLS-2, environments designated with a BLS-3 classification work with microbes that may carry potentially lethal diseases via respiratory transmission. Microbes can be either indigenous or exotic


Regulations and safety practices include:


-Adequate Personal Protective Equipment (PPE) - lab coats, gloves, mask, eye protection. Respirators may be required. 

-All microbe work must be performed using a Biological Safety Cabinet (BSC).

-A hands-free sink and eyewash must be available for disinfection.

-The laboratory has an airflow system that prevents the exhausted air from coming onto clean areas.


Facilities designated with BSL-3 classification include: Laboratories with two sets of self-closing and locking doors. Access to the laboratory is restricted/controlled at all times.

Bio-One certified technicians are caring, compassionate, and respectful of your privacy.

Biosafety Level 4 (BSL-4) 


Built upon BLS-3, environments designated with a BSL-4 classification work with the most dangerous and exotic bacteria and microbes. Diseases and infections caused by these microbes can be aerosol-transmitted and are usually fatal and impossible to treat or vaccinate against. 


Regulations and safety practices include:


-Mandatory change of clothing, before and after exiting the laboratory. A Post-exit shower is also mandatory.

-Decontamination of all used materials before exiting the laboratory.

-All microbe work must be performed using a Class III Biological Safety Cabinet (BSC).

-Adequate Personal Protective Equipment (PPE) - Full bodysuit with air supply and positive pressure system.


Facilities designated with BSL-4 classification include: Laboratories is in a separate building or isolated from the rest of the building. Laboratories must have dedicated air supply and exhaust air systems, as well as their own decontamination systems.


Bio-One or Orange can help you


Bio-One of Orange certified technicians follow strict guidelines and procedures to remove biohazardous waste from contaminated scenes. Whether it’s from blood and bodily fluids, medical waste, gases, and other biological agents, our specialists are trained to properly 

contain and dispose of the waste in an effective, timely manner that ensures safety for every party involved.


Bio-One of Orange is proud to serve the Orange County, California, and surrounding communities:


Aliso Viejo, Anaheim, Brea, Buena Park, Costa Mesa, Cypress, Dana Point, Fountain Valley, Fullerton, Garden Grove, Huntington Beach, Irvine, La Habra, La Palma, Laguna Beach, Laguna Hills, Laguna Niguel, Laguna Woods, Lake Forest, Los Alamitos, Mission Viejo, Newport Beach, Orange, Placentia, Rancho Santa Margarita, San Clemente, San Juan Capistrano, Santa Ana, Seal Beach, Stanton, Tustin, Villa Park, Westminster, and Yorba Linda!


Give us a call - 714-397-8375. Let Bio-One take care of you!

Request a service from your local Bio-One team.

Bio-One Of Orange

Bio-One Of Orange decontamination and biohazard cleaning services

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